Streaming potential measurements in -rat epithelial Na+ channel in planar lipid bilayers
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چکیده
منابع مشابه
Single ion-channel current measurements from rat brain synaptosomes in planar lipid bilayers.
Because of their small size and inaccessibility, direct electrical measurements of ionic conductances from mammalian brain presynaptic nerve terminals have not been possible. Measurements using voltage-sensitive fluorescent dyes, however, indicate that the membrane potential of a preparation enriched in pinched-off and resealed presynaptic nerve terminals (synaptosomes) depends principally on K...
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Purified bovine renal epithelial Na+ channels when reconstituted into planar lipid bilayers displayed a specific orientation when the membrane was clamped to -40 mV (cis-side) during incorporation. The trans-facing portion of the channel was extracellular (i.e., amiloride-sensitive), whereas the cis-facing side was intracellular (i.e., protein kinase A-sensitive). Single channels had a main sta...
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Single Na+ channels from rat skeletal muscle plasma membrane vesicles were inserted into planar lipid bilayers formed from neutral phospholipids and were observed in the presence of batrachotoxin. The batrachotoxin-modified channel activates in the voltage range -120 to -80 mV and remains open almost all the time at voltages positive to -60 mV. Low levels of tetrodotoxin (TTX) induce slow fluct...
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The hypothesis that actin interactions account for the signature biophysical properties of cloned epithelial Na(+) channels (ENaC) (conductance, ion selectivity, and long mean open and closed times) was tested using planar lipid bilayer reconstitution and patch clamp techniques. We found the following. 1) In bilayers, actin produced a more than 2-fold decrease in single channel conductance, a 5...
متن کاملSodium Channels in Planar Lipid Bilayers
Single channel currents of sodium channels purified from rat brain and reconstituted into planar lipid bilayers were recorded . The kinetics of channel gating were investigated in the presence of batrachotoxin to eliminate inactivation and an analysis was conducted on membranes with a single active channel at any given time . Channel opening is favored by depolarization and is strongly voltage ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.14.7651